proteome profiler human cell stress array kit  (R&D Systems)

Journal: Science Advances

Article Title: A dual chemodrug-loaded hyaluronan nanogel for differentiation induction therapy of refractory AML via disrupting lysosomal homeostasis

doi: 10.1126/sciadv.ado3923

Figure Lengend Snippet: ( A ) Wright-Giemsa staining of cells after 5-day treatment [Cis (0.45 μg/ml) and Dau (0.175 μg/ml)]. ( B ) Immunofluorescence staining of CD14 and the statistical data of the nucleus areas stained by DAPI. Scale bar, 20 μm. ( C ) Cathepsin B evaluation by a Magic Red assay kit and the relative integrated density of Magic Red in cytoplasm. Scale bar, 10 μm. ( D ) FACS analysis of the CD14 expression with or without NAC pretreatment ( n = 3). ( E ) Relative ROS in Kasumi-1 cells incubated with Cis + Dau and HA/Cis/Dau with or without NAC pretreatment ( n = 3). ( F ) Proteome profiler array. “*,” “#,” or “&” P < 0.05; “**,” “##,” or “&&” P < 0.01; *** P < 0.001; **** P < 0.0001. Con, control.

Article Snippet: Then, the Human Cell Stress Array Kit (R&D Systems, USA) was used to detect the differences among different treatment groups.

Techniques: Staining, Immunofluorescence, Expressing, Incubation, Control

Journal: Journal of Cosmetic Dermatology

Article Title: New Plant Extracts Exert Complementary Anti‐Hair Loss Properties in Human In Vitro and Ex Vivo Models

doi: 10.1111/jocd.16616

Figure Lengend Snippet: Phosphorylation of receptor tyrosine kinases (RTKs) and their downstream effectors in human follicle dermal papilla cells treated with a Silybum marianum extract (SME, 30 μg/mL, dissolved in dimethylsulfoxide [DMSO]) or DMSO for 1 h ( n = 2 donors, tests performed in duplicate for DMSO‐treated and SME‐treated cells from each donor). (a) EGFR and PDGFRβ, and (b) the downstream phosphokinases ERK1/2, GSK3α/β, Akt1/2/3, STAT5α and STAT5α/β. Data were obtained using the Human Proteome Profiler Phospho‐RTK and Phospho‐Kinase array kits, respectively. Each bar represents the mean ± SEM of SME/DMSO ratio.

Article Snippet: Growth factor receptor (GFR) signaling pathways were analyzed using two Human Proteome Profiler antibody array kits (R&D Systems, USA) to simultaneously detect the phosphorylation of 49 receptor tyrosine kinases (Proteome Profiler Human Phospho‐RTK Array Kit) and of 43 kinases and two related total proteins (Proteome Profiler Human Phospho‐Kinase Array Kit).

Techniques: Phospho-proteomics